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Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres BABT
Chen,Meng-Yan; Zheng,Wei; Dong,Qiu-Yue; Li,Zhen-Hua; Shi,Lu-E; Tang,Zhen-Xing.
In this work, alginate-whey protein was used as wall materials for encapsulating Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The characteristics of encapsulated and free L. bulgaricus showed that the free L. bulgaricus lost viability after 1 min exposure to simulated gastric fluid (SGF) at pH 2.0 and 2.5. However, the viability of encapsulated L. bulgaricus did not decrease in SGF at pH 2.5 for 2 h incubation. The viable numbers of encapsulated L. bulgaricus decreased less than 1.0 log unit for 2 h incubation in SGF at pH 2.0. For bile stability, only 1.2 log units and 2.0 log units viability of the encapsulated L. bulgaricus was lost in 1 and 2% bile for 1 h exposure, respectively, compared with no survival of free L. bulgaricus under the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Alginate; Whey protein; Probiotics; Stability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500736
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Characterization of powder from the permeate of yacon extract by ultrafiltration and dehydrated by spray drying Ciência e Agrotecnologia
Brites,Marcela Lazzare; Meira,Stela Meister; Brandelli,Adriano; Noreña,Caciano Zapata.
ABSTRACT Yacon root is a functional food which contains antioxidants and prebiotics compounds. This study aimed to evaluate the physical, chemical and prebiotic characteristics of a yacon extract powder obtained by ultrafiltration (UF) with membranes of 10 and 30 kDa and encapsulation of the resulting permeate by spray drying. Drying air temperatures of 140 and 160 ºC and concentrations of gum arabic of 10 and 15% were tested. The samples had solubility values ​​greater than 90% while the hygroscopicity decreased with increasing gum concentration and drying temperature. Electron microscopy showed a strong tendency to agglomeration of smaller particles around the larger ones, mainly at a temperature of 140 ºC. Regarding color, the parameter L* showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Inulin; Prebiotic activity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000500585
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Compatibility of polymers to fungi Beauveria bassiana and Metarhizium anisopliae and their formulated products stability Agronomy
Wenzel Rodrigues, Inajá Marchizeli; Batista Filho, Antonio; Giordano, Isabella Barichello; Denadae, Bárbara Evelin; Fernandes, João Batista; Forim, Moacir Rossi.
Stability during storage is a limiting factor in the development and use of bioinsecticides. This study aims to evaluate the compatibility of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana with components used in encapsulated formulations and to evaluate shelf-life in formulations with B. bassiana. Sodium alginate and maltodextrin, hemicellulose, and dimethyl sulfoxide solvents were evaluated. Compatibility was evaluated by mixing the components with PDA, and the parameters evaluated were vegetative growth, sporulation and viability. The formulations of fungi were prepared in various concentrations of sodium alginate and then added drop-wise to calcium chloride. To evaluate the stability, formulations were stored at 26.5, 4.0, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial control; Formulations; Entomopathogens; Encapsulation; Shelf-life; Microbiology..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32903
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Controlled Release of TBH Herbicide Encapsulated on Ca-ALG Microparticles: Leaching and Phytointoxication Plants Planta Daninha
MACEDO,D.F.; DOURADO JR.,S.M.; NUNES,E.S.; MARQUES,R.P.; MORETO,J.A..
ABSTRACT: The aim of this work was to demonstrate a detailed study of the controlled release of the herbicide Tebuthiuron (TBH) encapsulated in microparticles of calcium alginate (Ca-ALG), to evaluate the phytotoxicity in different concentrations of herbicide (4, 6 and 8 g L-1), and their correlation with the depth of leaching using bioindicator plants. The Ca-ALG microparticles were prepared from the crosslinking of sodium alginate by Ca++ containing varied amounts of TBH supplied in calcium chloride (CaCl2) aqueous solution. The results showed that TBH herbicide, when encapsulated, leached to shallow depths relative to the conventional, non-encapsulated herbicide (which moved to a depth of 40-50 cm). The concentration of 4 g L-1 was the one that leached...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pesticides; Biodegradable polymer; Environmental impact; Encapsulation; Agriculture.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100266
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Cotesia flavipes (CAM) (Hymenoptera: Braconidae) Supresses Immune Responses In Diatraea flavipennella (BOX) (Lepidoptera: Crambidae) Anais da ABC (AABC)
PASSOS,ELIANA M. DOS; WANDERLEY-TEIXEIRA,VALÉRIA; MARQUES,EDMILSON J.; TEIXEIRA,ÁLVARO A.C.; BRAYNER,FÁBIO A..
The present research aimed to elucidate which aspects of immune responses in Diatraea flavipennella are suppressed by the parasitoid Cotesia flavipes, thus, ensuring parasitism success. We investigated the presence of apoptosis in fat body cells through the TUNEL technique. According to the results, reduced levels of nitric oxide and phenoloxidase activity were observed in larvae parasitized for three days, and reduced total number of hemocytes, after three and seven days. An increase in plasmatocytes and decrease in spherulocytes numbers were observed in the differential count on the third day of parasitism. The number of melanized microspheres in parasitized larvae was low and indicated less intense melanization. The ultrastructural analysis confirmed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Hemocytes; Humoral response; Parasitoid; Sugarcane Moth Borer (Diatraea saccharalis).
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402013
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Defense reactions of Dermatobia hominis (Diptera: Cuterebridae) larval hemocytes Biocell
Faraldo,Ana Carolina; Lello,Edy.
The defense reactions against biological (Histoplasma capsulatum and Escherichia coli) and non-biological materials (China ink and nylon thread) were tested in vivo in third instar larvae of Dermatobia hominis. The cellular defense performed by larval hemocytes was observed under electron microscopy. China ink particles were phagocytosed by granular cells 5 h after injection. E. coli cells were internalized by granular cells as early as 5 min after injection and totally cleared 180 min post-injection, when many hemocytes appeared disintegrated and others in process of recovering. H. capsulatum yeasts provoked, 24 h after being injected, the beginning of nodule formation. Nylon thread was encapsulated 24 h after the introduction into the hemocoel. Our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatobia Hominis; Hemocytes; Phagocytosis; Nodule Formation; Encapsulation.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200005
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Development of artificial sugarcane seed CP-54 from three cultivars (cv MEX 69-290; cv MEX 68-P-23; cv. CP 72-2086) using polymers in Tabasco, Mexico Acta Agron. (Palmira)
Álvarez-Sánchez,Geiner F; Arias-de la Cruz,Heidi L; Salgado-García,Sergio; Córdova-Sánchez,Samuel; Ortiz-Laurel,Hipólito; Castelán-Estrada,Mepivoseth; García-de la Cruz,Rubén; Castañeda-Ceja,Raúl.
Abstract Precision mechanized sowing of sugarcane has been delayed due to germination failures caused in sowing process by pieces of sugarcane, generating economic losses by reducing production. The aim of this study was to evaluate different polymers and cultivars of sugarcane to elaborate artificial seed CP-54, seed germination and quality of sugarcane plants. An experiment was carried out in two stages in experimental field of the Colegio de Postgraduados Campus Tabasco, Mexico. Results indicate that the best physical stage of artificial seed CP-54 was obtained in Mex 69-290, Mex 68-P-23 and CP 72-2086 cultivars encapsulated with starch polymers and sodium alginate. Artificial seed CP-54 germination was 100 and 84% encapsulated with starch and alginate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Precision agriculture; Saccharum spp.; Seed germination; Buds; Sugarcane response.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000100094
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Encapsulation of lycopene using spray-drying and molecular inclusion processes BABT
Nunes,Itaciara Larroza; Mercadante,Adriana Zerlotti.
This study aimed to obtain encapsulated lycopene in a powder form, using either spray-drying or molecular inclusion with beta -cyclodextrin ( beta -CD) followed by freeze-drying. The encapsulation efficiency using spray-drying ranged from 94 to 96%, with an average yield of 51%, with microcapsules showing superficial indentations and lack of cracks and breakages. Lycopene- beta -CD complexes were only formed at a molar ratio of 1:4, and irregular structures of different sizes that eventually formed aggregates, similar to those of beta -CD, were observed after freeze-drying. About 50% of the initial lycopene did not form complexes with beta -CD. Lycopene purity increased from 96.4 to 98.1% after spray-drying, whereas lycopene purity decreased from 97.7 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopene; Encapsulation; Spray-drying; Gum arabic; Inclusion complex; Beta -cyclodextrin.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500018
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GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION Rev. Bras. Frutic.
PEREIRA,JÚLIO CÉSAR GOMES; ROCHA,SELMA SILVA; LONDE,LUCIANA CARDOSO NOGUEIRA; MOTA,MARCELA CAROLINE BATISTA DA; ALVES,PABLO FERNANDO SANTOS; VIANA,WANDER SILVA.
ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Micropropagation; Musa sp.; Growth regulators.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000500704
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Hepatic toxicity caused by PLGA-microspheres containing usnic acid from the lichen C ladonia substellata (AHTI) during pregnancy in Wistar rats Anais da ABC (AABC)
MARINHO,KETSIA S.N.; ANTONIO,ERIVALDO A.; SILVA,CAMILLA V.N.S.; SILVA,KEILA T. DA; TEIXEIRA,VALÉRIA W.; AGUIAR JUNIOR,FRANCISCO C.A. DE; SANTOS,KATHARINE R.P. DOS; SILVA,NICÁCIO H. DA; SANTOS,NOEMIA P.S..
ABSTRACT This study aimed to evaluate the teratogenic and hepatotoxic potential of the usnic acid encapsulated into PLGA-microspheres. In total, 12 female Wistar rats in pregnancy were randomly distributed in the control group (n= 6) that received 1.0 mL of physiological solution and treatment group (n= 6) that received 25 mg/kg of encapsulated usnic acid by oral administration. All females were euthanized at day 20 of pregnancy and their fetuses were removed and analyzed. During the pregnancy was observed a reduction in weight gain. There was no difference in serum transaminases levels analyzed as well as any difference in liver weight in both groups. The histomorphometric analysis of the liver from the treatment group revealed an increase in number of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Fetotoxicity; Hepatotoxicity; Secondary metabolite.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301073
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In Vitro Mid-Term Conservation of Acorus calamus L. via Cold Storage of Encapsulated Microrhizome BABT
Quraishi,Afaque; Mehar,Snigdha; Sahu,Durga; Jadhav,Shailesh Kumar.
ABSTRACT In vitro rhizome production, encapsulation and cold storage of Acorus calamus were attempted for its propagation and ‘true-to-type’ conservation. Shoot cultures were initiated using underground rhizome buds, on 6-benzyladenine (BA) containing Murashige and Skoog (MS) medium. Maximum microrhizome production was observed in presence of 33.3 µM BA, on modified MS medium containing 6% sucrose, 100 mg/L citric acid and 1 g/L polyvinyl pyrrolidone-40. Synthetic seeds were produced from regenerated microtubers by encapsulation in calcium alginate beads. These synthetic seeds were stored in complete darkness at 100C temperature for different durations for mid-term conservation. After cold storage, synthetic seeds were re-cultured in vitro, 100% survival...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aromatic plant; Encapsulation; Sweet Flag; Synthetic seeds; Plant tissue culture.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100412
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Infestation of the cupped oysters Crassostrea angulata, C. gigas and their first-generation hybrids by the copepod Myicola ostreae: differences in susceptibility and host response ArchiMer
Batista, F. M.; Boudry, Pierre; Dos Santos, A.; Renault, Tristan; Ruano, F..
We Studied the prevalence and intensity of the parasitic copepod Myicola ostreae in 2 closely related oysters Crassostrea angulata and C. gigas and their F1 hybrids. The effects on host and host reaction were also analysed to better understand host-parasite relationships between copepods and bivalve molluscs. Full reciprocal crosses were carried out between C. angulata and C. gigas and the progenies were reared in the wild in Ria Formosa Lagoon (Portugal), allowing natural infestation by M. ostreae. Prevalence and intensity were significantly higher in C. angulata than in C. gigas. The parasite level of F1 hybrids was similar to C. angulata and significantly higher than in C. gigas. The results of our study support a hypothesis of dominantly inherited...
Tipo: Text Palavras-chave: Encapsulation; Host reaction; Host parasite relationship; Dominance inherence; Parasitism; Hybridization; Copepods; Oysters.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6442.pdf
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Inhibition of listeria monocytogenes in minas frescal cheese by free and nanovesicle-encapsulated nisin BJM
Malheiros,Patrícia da Silva; Daroit,Daniel Joner; Brandelli,Adriano.
The effectiveness of free and nanovesicle-encapsulated nisin to control Listeria monocytogenes in Minas Frescal cheese was investigated. Commercial nisin was encapsulated into liposomes of partially purified soy lecithin. Free (0.1 mg/mL and 0.25 mg/mL) and nanovesicle-encapsulated nisin (0.25 mg/mL) were applied onto the surface of cheese samples, and L. monocytogenes was inoculated before incubation at 6-8°C for 28 days. A bactericidal effect was observed with 0.25 mg/mL free nisin; a bacteriostatic effect was observed for liposome-encapsulated nisin and 0.1 mg/mL free nisin. Free nisin was more efficient than nisin-loaded liposomes in controlling L. monocytogenes. Possible reasons for this behavior, and also the significance of nisin to soft cheeses are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nisin; Liposome; Encapsulation; Cheese; Listeria monocytogenes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400024
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Liposomes: from biophysics to the design of peptide vaccines BJMBR
Frézard,F..
Liposomes (lipid-based vesicles) have been widely studied as drug delivery systems due to their relative safety, their structural versatility concerning size, composition and bilayer fluidity, and their ability to incorporate almost any molecule regardless of its structure. Liposomes are successful in inducing potent in vivo immunity to incorporated antigens and are now being employed in numerous immunization procedures. This is a brief overview of the structural, biophysical and pharmacological properties of liposomes and of the current strategies in the design of liposomes as vaccine delivery systems.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Liposome; Immunoadjuvant; Immunostimulant; Vaccine; Encapsulation.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200006
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Microencapsulation and Storage Stability of Lycopene Extracted from Tomato Processing Waste BABT
Ranveer,Rahul C.; Gatade,Abhijeet A.; Kamble,Harshwardhan A.; Sahoo,Akshya K..
ABSTRACT The aim of this study was to optimize the encapsulation of lycopene using response surface methodology and to determine its stability. The lycopene was extracted from tomato processing industry waste. The extracted pigment was purified by crystallization method. The effect of different process parameters, viz, core to wall ratio, sucrose to gelatin and inlet temperature on encapsulation efficiency (EE) and encapsulation yield (EY) were studied. Structural study of encapsulated material was carried by using scanning electron microscope (SEM). The samples with and without encapsulation were stored under different conditions such as the presence and absence of air, sunlight, at room temperature and under refrigeration. Highest EE (92.6 ± 0.86) and EY...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopene; Encapsulation; Encapsulation efficiency; Encapsulation yield; RSM.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600953
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Microencapsulation of pequi pulp by spray drying: use of modified starches as encapsulating agent REA
Santana,Audirene A.; Oliveira,Rafael A. de; Kurozawa,Louise E.; Park,Kil J..
The aim of this study was to evaluate the microencapsulation of pequi pulp by spray drying. A central composite rotational design was used in order to evaluate the effect of the independent variables: inlet air temperature, surfactant concentration and modified starch concentration. The dependent variables were assumed as yield of the process and the product features microencapsulated. A selection of the best process condition was performed to obtain the best condition of a product with the highest vitamin C and carotenoids content. Powders showed moisture content below 2%. The experimental values of hygroscopicity, yield, water activity, total carotenoids and vitamin C powders ranged from 7.96 to 10.67 g of adsorbed water/100g of solids, 24.34 to 49.80%,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caryocar brasiliense; Spray drying; Experimental design; Encapsulation; Morphology.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162014000500017
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Novel methods for the encapsulation of meglumine antimoniate into liposomes BJMBR
Frézard,F.; Michalick,M.S.M.; Soares,C.F.; Demicheli,C..
The antimonial drug, meglumine antimoniate, was successfully encapsulated in dehydration-rehydration vesicles and in freeze-dried empty liposomes (FDELs). High encapsulation efficiencies (from 28 to 58%) and low weight ratios of lipids to encapsulated antimony (from 1:0.15 to 1:0.3) were achieved. These formulations, contrary to those obtained by conventional methods, can be stored as intermediate lyophilized forms and reconstituted just before use. The efficacy of FDEL-encapsulated meglumine antimoniate was evaluated in hamsters experimentally infected with Leishmania chagasi. A significant reduction of liver parasite burdens was observed in animals treated with this preparation, when compared to control animals treated with empty liposomes. In contrast,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Liposomes; Glucantime; Meglumine antimoniate; Leishmaniasis; Encapsulation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700016
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Oviposition sequence and offspring of mated and virgin females of Cotesia flavipes (Hymenoptera: Braconidae) parasitizing Diatraea saccharalis larvae (Lepidoptera: Crambidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Scaglia,M.; Chaud-Netto,J.; Brochetto-Braga,M. R.; Ceregato,S. A.; Gobbi,N.; Rodrigues,A..
Large scale mass rearing of natural enemies has been a mean of improving biological control in the sugarcane intensive agriculture. Among them, Cotesia flavipes, a gregarious koinobiont endoparasitoid, was imported by Brasil to control caterpillars of the sugarcane borer Diatraea saccharalis. The C. flavipes larval development depends on its association with polydnavirus, which blocks the host defense reaction. To verify if the oviposition sequence (1st, 2nd or 3rd) and the female condition (mated or virgin) interfere in the number of C. flavipes descendents, 4th instar caterpillars of D. saccharalis were parasitized. Analysis of the data showed that: a) there is an inverse correlation between the parasitism efficiency and the host reaction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cotesia flavipes; Diatraea saccharalis; Oviposition sequence; Encapsulation; Chrysalises; Parasitism.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300007
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Preparation and cytotoxicity of cisplatin-containing liposomes BJMBR
Carvalho Júnior,A.D.; Vieira,F.P.; De Melo,V.J.; Lopes,M.T.P.; Silveira,J.N.; Ramaldes,G.A.; Garnier-Suillerot,A.; Pereira-Maia,E.C.; De Oliveira,M.C..
We encapsulated cisplatin into stealth pH-sensitive liposomes and studied their stability, cytotoxicity and accumulation in a human small-cell lung carcinoma cell line (GLC4) and its resistant subline (GLC4/CDDP). Since reduced cellular drug accumulation has been shown to be the main mechanism responsible for resistance in the GLC4/CDDP subline, we evaluated the ability of this new delivery system to improve cellular uptake. The liposomes were composed of dioleoylphosphatidylethanolamine (DOPE), cholesteryl hemisuccinate (CHEMS), and distearoylphosphatidylethanolamine-polyethyleneglycol 2000 (DSPE-PEG2000) and were characterized by determining the encapsulation percentage as a function of lipid concentration. Among the different formulations,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cisplatin; Liposomes; Encapsulation; Cytotoxicity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800017
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Promoting fruit seedling growth by encapsulated microorganisms Rev. Bras. Frutic.
Santos,Carlos Henrique Barbosa; Martins,Antonio Baldo Geraldo; Rigobelo,Everlon Cid; Teixeira,Gustavo Henrique de Almeida.
Abstract The use of microorganisms capable of promoting plant growth has been accepted as an alternative to reducing the use of chemical fertilizers. The aim of this study was to evaluate the inoculation of plant growth promoting microorganisms in seedlings of fruit species, verifying the interaction of the inoculums with encapsulating agents such as clay and alginate. Microbial inoculums contained the following species: Azospirillum brasilense, Burkolderia cepacia, Bacillus thuringienses, Bacillus megaterium, Bacillus cereus,Bacillus subtilis, Tricoderma spp. and isolate 411. The fruit species evaluated were: Myrciaria cauliflora (DC.) O. Berg; Myrciaria glazioviana (Kiaersk.) G. Barros &Sobral; Myrciaria dubia (Kunth) Mc Vaugh; Eugenia brasiliensis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fruit plants; Development; Encapsulation; Plant-growth promoting agents.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000301001
Registros recuperados: 25
Primeira ... 12 ... Última
 

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